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[乙肝] 非肿瘤内 VS 肿瘤内乙肝病毒DNA影响和复制在肝细胞肝癌中

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发表于 2016-8-29 13:50:40 | 显示全部楼层 |阅读模式

Impact of non-neoplastic vs intratumoural hepatitis B viral DNA and replication on hepatocellular carcinoma recurrence

Background:

This study aims to determine the impact of intracellular hepatitis B virus (HBV) DNA, covalently closed circular DNA (cccDNA) and viral replicative activity in both tumour and non-neoplastic liver on prognosis and to determine the relationship of viral replicative activity and Ishak fibrosis in predicting outcome following resection.

Methods:

A total of 99 prospectively enrolled patients treated with primary liver resection for HBV-HCC are included. Intracellular HBV DNA and cccDNA were quantitated by real-time PCR. The RNA-sequencing (RNA-seq) was performed in a subset of 21 patients who had either minimal liver fibrosis (Ishak stages 0–2) or end-stage fibrosis (Ishak stage 6).

Results:

Tumour tissue contained a lower cccDNA copy number compared with paired non-neoplastic liver, and larger tumours (>3 cm) had less cccDNA compared with small tumours (less than or equal to3 cm). High viral replicative activity in non-neoplastic liver was associated with higher HCC recurrence rate independent of Ishak fibrosis stage. Genes correlated with viral replicative activity in non-neoplastic liver (620 genes) were distinct from those associated with end-stage fibrosis (1226 genes). Genes associated with viral replicative activity were preferentially distributed in regions on chr3, chr16 and chr19.

Conclusions:

Viral replicative activity in non-neoplastic liver is associated with HCC recurrence through mechanisms that are distinct from and independent of Ishak fibrosis stage.

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 楼主| 发表于 2016-8-29 14:00:54 | 显示全部楼层
背景:

这项研究的目的是确定细胞内乙型肝炎病毒(HBV)DNA的影响,共价闭合在肿瘤和非肿瘤性肝环状DNA(cccDNA的)和病毒复制的活性对预后,并确定病毒复制的活性和伊沙克纤维化的关系预测下切除术的结果。

方法:

共有99原发性肝癌切除HBV-肝癌治疗的前瞻性纳入的患者都包括在内。通过实时PCR的细胞内HBV DNA和cccDNA的进行定量。该RNA测序(RNA-SEQ),在21例谁不是最小的肝纤维化(伊沙克阶段0-2)或终末期纤维化(伊沙克阶段6)的一个子集进行。

结果:

肿瘤组织包含低级的cccDNA拷贝数与配对非肿瘤性肝相比,用小肿瘤(小于或等于TO3厘米)相比,较大的肿瘤(> 3厘米)有较少的cccDNA。在非肿瘤性肝病毒高复制活性与HCC复发率无关伊沙克纤维化分期较高有关。与非肿瘤性肝(620个基因)的病毒复制活性相关的基因是从终末期纤维化(1226个基因)相关的那些不同。与病毒复制活动相关的基因被优先分布在CHR 3,chr16和chr19地区。

结论:

在非肿瘤性肝病毒复制活动与HCC复发通过从不同的独立的伊沙克纤维化阶段的机制有关。
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