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【资讯翻译】Human beta-defensin 1, 2 and 3 production by amniotic epith...

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发表于 2016-6-13 11:34:48 | 显示全部楼层 |阅读模式
Human beta-defensin 1, 2 and 3 production by amniotic epithelial cells with respect to human papillomavirus (HPV) infection, HPV oncogenic potential and the mode of delivery.
BACKGROUND:
Human beta-defensins (HBD) produced by human amniotic epithelial cells (HAEC) co-create an innate antiviral immune response in the materno-placento-fetal unit. Oncogenic potential of HPV may reflect its ability to avoid immune recognition. In this study we assessed the risk of HAEC infection with human papillomavirus (HPV) in relation to the type of labor and the impact of the oncogenic potential of HPV on HBD production in HAEC.

METHODS:
A comparative analysis [HPV(+) vs. HPV(-)HAEC] of the production of HBD were performed. HAEC were isolated from placentas of 116 HPV(+) and 36 HPV(-) parturients (groups I and II, respectively) using trypsin-based method. The cases of premature rupture of membranes (PROM), natural labors (NL) and cesarean sections (CS) were analysed in respective subgroups. High-risk (HR-HPV) and low-risk (LR-HPV) genotypes of HPV in cervical smears and HAEC were identified using the Roche Linear Array® HPV Genotyping Test. HBD-1,-2,-3 concentrations in the HAEC culture supernatant were assessed using ELISA.

RESULTS:
The highest percentage (42.1%) of HPV transmission to HAEC occurred in PROM, an intermediate value was observed after NL (38.5%), and the lowest (25.6%) after CS. The mean concentrations of HBD-2 and HBD-3 in group I were up to 3.1- and 2.8-fold higher (p < 0.05), respectively. The mean concentration of HBD-2 was higher (p < 0.05) in LR-HPV infection compared with HR-HPV.

CONCLUSIONS:
The course of labor and the mode of delivery influence the risk of HPV transmission to the HAEC. HPV infection upregulates HBD-2 and HBD-3 production in HAEC. Smaller increases in HBD-2 level after HR-HPV infection as compared to LR-HPV may affect cancerogenesis. Therapeutic potential of HBD-2 for HR-HPV infection should be assessed in future studies.

http://www.ncbi.nlm.nih.gov/pubmed/27289038


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发表于 2016-6-13 13:13:06 | 显示全部楼层
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发表于 2016-6-15 09:59:08 | 显示全部楼层

人羊膜上皮细胞制造的人β-防御素 1、2和3与人乳头瘤病毒(HPV)感染、HPV的潜在致癌性和分娩模式的关系

背景:
由人羊膜上皮细胞(HAEC)制造的人β-防御素(HBD)在孕妇-胎盘-胎儿单位中共同制造一种先天的抗病毒免疫反应。HPV的潜在致癌性可能反映了其躲避免疫识别的能力。在这项研究中,我们评估了由人乳头瘤病毒(HPV)造成的HAEC感染与分娩方式的关系,以及HPV的潜在致癌性对于HBD在HAEC中的制造的影响。

方法:
进行一项[HPV(+)对HPV(-)HAEC]在HBD制造上的对比分析。在116名HPV(+)产妇和36名HPV(-)产妇中,使用基于胰蛋白酶的方法将HAEC从胎盘中分离(分别为组I和组II)。对于胎膜早破(PROM)的情况,分别对自然分娩(NL)和剖宫产(CS)在各组的亚组中进行了分析。使用罗氏Linear Array® HPV基因分型检测对宫颈刮片中的HPV高危(HR-HPV)和低危(LR-HPV)基因型进行测定,用ELISA 评估HAEC培养上清液中的HBD-1、-2、-3。

结果:
HPV转移到HAEC的最高比例(42.1%)出现在PROM中,在NL后观察到中间值(38.5%),CS后观察到最低值(25.6%)。在组I中,HBD-2和HBD-3的平均浓度分别可以达到3.1倍和2.8倍以上(P <0.05)。在LR-HPV感染中HBD-2的平均浓度高于HR-HPV(P <0.05)。

结论:
分娩过程和分娩模式影响HPV转移到HAEC的风险。在HAEC中的HPV感染使HBD-2和HBD-3的水平上调。与LR-HPV相比,HR-HPV感染后HBD-2的提高较小,可能对癌的形成有影响。在未来的研究中应该对HBD-2在HR-HPV感染中的治疗潜能进行评估。

http://www.ncbi.nlm.nih.gov/pubmed/27289038

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 楼主| 发表于 2016-6-15 11:24:44 | 显示全部楼层
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