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【文献翻译】High-Sensitivity Sandwich ELISA for Plasma NT-proUcn2: Plas...

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发表于 2016-6-2 15:17:04 | 显示全部楼层 |阅读模式
High-Sensitivity Sandwich ELISA for Plasma NT-proUcn2: Plasma Concentrations and Relationship to Mortality in Heart Failure

BACKGROUND: Urocortin 2 (Ucn2) has powerful hemodynamic, renal, and neurohormonal actions and likely participates in normal circulatory homeostasis and the compensatory response to heart failure (HF). A validated assay for endogenous circulating Ucn2 would facilitate investigations into Ucn2 physiology and elucidate its derangement and potential as a biomarker in heart disease.

METHOD: We developed a chemiluminescence-based sandwich ELISA to measure plasma N-terminal (NT)-proUcn2 in non-HF patients (control; n = 160) and HF patients with reduced (HFREF; n = 134) and preserved (HFPEF; n = 121) left ventricular ejection fraction (LVEF).

RESULTS: The ELISA had a limit of detection of 8.47 ng/L (1.52 pmol/L) and working range of 23.8–572 ng/L. Intra- and interassay CV and total error were 4.8, 16.2, and 17.7%, respectively. The median (interquartile range) plasma NT-proUcn2 concentration in controls was 112 (86–132) ng/L. HFREF, HFPEF, and all HF plasma concentrations were significantly increased [117 (98–141) ng/L, P = 0.0007; 119 (93–136) ng/L, P = 0.0376, and 119 (97–140) ng/L, P = 0.001] compared with controls but did not differ significantly between HFREF and HFPEF. NT-proUcn2 was modestly related to age (r = 0.264, P = 0.001) and cardiac troponin T (r = 0.258, P = 0.001) but not N-terminal pro-B-type natriuretic peptide, body mass index, LVEF, or estimated glomerular filtration rate. On multivariate analysis, plasma NT-proUcn2 was independently and inversely related to 2-year mortality in HF.

CONCLUSIONS: The validated ELISA measured human NT-proUcn2 in plasma and showed modest but significant increases in HF patients compared with controls. In HF, the unusual inverse relationship between plasma NT-proUcn2 and 2-year mortality portends potential prognostic value but requires further corroboration.

链接:http://www.clinchem.org/content/62/6/856.abstract

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发表于 2016-6-3 12:38:25 | 显示全部楼层
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发表于 2016-6-3 14:13:18 | 显示全部楼层
血浆NT-proUcn2高灵敏度的夹心ELISA测试:血浆浓度及其与心力衰竭死亡率的关系

       背景:尿皮素2(ucn2)具有很强的血流动力学,肾和神经激素样作用,并可能参与正常的循环代谢和心脏衰竭(HF)的代偿反应。有效的内生循环ucn2shui检测有助于研究ucn2生理作用及阐明其紊乱及作为心脏病的生物标志物潜在可能性。

       方法:我们开发了一个基于化学发光的夹心ELISA法,用于测定非心衰患者(对照;n = 160)和左室射血分数(LVEF)减少(HFREF;n = 134)和保持(HFPEF;n = 121)的HF患者血浆氨基末端(NT)-Ucn2原。
       结果:ELISA检测限为8.47 ng/L(1.52 pmol/L),工作范围为23.8–572 ng/L。批内、批间变异系数和总误差分别为4.8,16.2,和17.7%。对照组血浆nt-proucn2浓度的中位数(四分间距)为112(86–132)ng/L. 对照组相比,HFREF、EF和所有HF病人血浆nt-proucn2浓度显著增加[ 117(98–141)ng/L,P = 0.0007;119(93–136)ng/L,P = 0.0376,和119(97–140)ng/L,P = 0.001 ],但是HFREF和EF组之间的差异不显著。nt-proucn2与年龄(R = 0.264,P = 0.001)和心肌肌钙蛋白T(r = 0.258,P = 0.001)具有一定的相关性,而与N末端B型钠尿肽、身体质量指数、左室射血分数,或估算的肾小球滤过率不相关。在多变量分析中,血浆nt-proucn2与 HF2年死亡率呈现独立负相关。
       结论:利用验证的ELISA测定血浆人nt-proucn2,并发现与对照组相比,HF病人血浆人nt-proucn2呈现增加不大但显著的变化。HF病人血浆nt-proucn2与2年死亡率不寻常的负相关性预示着其潜在的程序价值,但仍需要进一步佐证。
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