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【资讯翻译】Circulating melanoma exosomes as diagnostic and prognosis b...

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发表于 2016-1-12 14:34:39 | 显示全部楼层 |阅读模式
Circulating melanoma exosomes as diagnostic and prognosis biomarkers

Background
Malignant melanoma is an aggressive cancer with an increasing incidence. Exosomes are actively secreted microvesicles, whose characteristics reflect those of the cell they are originated in. The aim of this study was to identify and evaluate the presence of the melanoma biomarkers MIA, S100B and tyrosinase-related protein 2 (TYRP2) in exosomes and their potential clinical utility.
Methods
Serum samples were obtained from stage IV melanoma patients, melanoma-free patients and healthy controls. Exosomes were precipitated and TYRP2, MIA and S100B concentrations were quantified in serum, exosomes, and exosome-free serum.
Results
Both MIA and S100B were detected in exosomes and correlated significantly with serum concentrations (S100B: r = 0.968; MIA: r = 0.799; p < 0.001). MIA and S100B concentrations in exosomes were significantly higher in melanoma patients than in healthy controls and disease-free patients. However, TYRP2 concentrations in exosomes did not differ between these three groups. ROC curves analysis rendered AUCs for MIA of 0.883 (p < 0.01) and of 0.840 for S100B (p < 0.01). Patients with exosome MIA concentration higher than 2.5 μg/L showed shorter median survival related to those with lower level (4 versus 11 months; p < 0.05).
Conclusions
MIA and S100B can be detected in exosomes from melanoma patients and their quantification presents diagnostic and prognostic utility.

信源地址:http://www.sciencedirect.com/science/article/pii/S0009898115301005

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发表于 2016-1-13 19:17:47 | 显示全部楼层
领了。。。。。。。

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发表于 2016-1-14 00:32:53 | 显示全部楼层
循环胞外体作为黑色素瘤诊断和预后的生物标志物

       背景:恶性黑色素瘤是一种侵略性肿瘤,其发病率越来越高。胞外体是一种主动分泌的微囊泡,他的特征反映其起源于何种细胞。本研究的目的是确定和评估胞外体中黑色素瘤标志物的存在,包括MIA,S100B和酪氨酸酶相关蛋白2(Tyrp2)等,及胞外体中的这些标记物的潜在临床应用价值。
      方法:收集黑色素瘤IV期患者,非黑色素瘤患者,和健康对照人群的血清样品。沉淀获得胞外体;对血清,胞外体和无胞外体血清中的TYRP2,MIA和S100B的浓度进行定量。
      结果:在胞外体中检测到了MIA和S100B,并与血清浓度呈显著相关(S100B: r = 0.968; MIA: r = 0.799; p < 0.001)。黑色素瘤患者胞外体中MIA和S100B浓度显著高于健康对照人群和非黑色素瘤患者。然而,三组人群血清胞外体中TYRP2的浓度没有无差异。MIA的ROC曲线分析所得峰下面积(AUC)为0.833(p < 0.01),S100B的ROC曲线分析所得峰下面积(AUC)为0.840(p < 0.01)。胞外体中MIA浓度高于2.5μg/L的病人的平均生存期比胞外体中MIA水平低的病人短(前者为4个月,后者为11个月,p < 0.05)。
      结论:在MIA和S100B黑色素瘤患者胞外体中检测到了MIA和S100B,对它们的量化具有诊断和预后的作用。

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 楼主| 发表于 2016-1-14 11:17:32 | 显示全部楼层
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