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【资讯翻译】Circulating Tumor Cell Enumeration with a Combination of Ep...

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发表于 2015-1-7 10:18:29 | 显示全部楼层 |阅读模式
Circulating Tumor Cell Enumeration with a Combination of Epithelial Cell Adhesion Molecule– and Cell-Surface Vimentin–Based Methods for Monitoring Breast Cancer Therapeutic Response

BACKGROUND: Detection, isolation, and enumeration of circulating tumor cells (CTCs) from cancer patients has become an important modality in clinical management of patients with breast cancer. Although CellSearch, an epithelial cell adhesion molecule (EpCAM)-based method that is used to isolate epithelial CTCs, has gained prominence, its inability to detect mesenchymal CTCs from breast cancer patients raises concerns regarding its utility in clinical management.
METHODS: To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma tumors. In the present study, we tested the sensitivity and specificity of detecting CTCs from blood collected at a random time during therapy from each of 58 patients with metastatic breast cancer by use of 84-1 (a monoclonal antibody against CSV to detect epithelial/mesenchymal-transition CTCs) and CellSearch methods. Additionally, we tested the possibility of improving the sensitivity and specificity of detection by use of additional parameters including nuclear EpCAM localization and epithelial mesenchymal ratios.
RESULTS: CTC counts with CSV were significant (P = 0.0053) in differentiating populations responsive and nonresponsive to treatment compared with CTC counts with CellSearch (P = 0.0564). The specificity of CTC detection was found to be highest when the sum of CTC counts from the 2 methods was above a threshold of 8 CTCs/7.5 mL.
CONCLUSIONS: The sum of CTC counts from the CellSearch and CSV methods appears to provide new insights for assessment of therapeutic response and thus provides a new approach to personalized medicine in breast cancer patients.

信源地址:http://www.clinchem.org/content/61/1/259.abstract

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发表于 2015-1-7 21:09:21 | 显示全部楼层
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发表于 2015-1-7 22:38:26 | 显示全部楼层
基于循环肿瘤细胞计数联合表面细胞粘附分子以及细胞表面波形蛋白的方法监测乳腺癌的治疗反应


背景: 检测,分离以及从病人体内分离循环肿瘤细胞的计数已经成为临床管理乳腺癌病人的重要方法。尽管基于表面粘附分子(EpCAM)分离上皮肿瘤循环细胞的方法Cellsearch已经占据主导地位,但是其不能诊断乳腺癌患者间叶细胞循环肿瘤细胞是在临床管理中引发担忧的一个方面。

方法: 为了解决技术上的瓶颈,我们最近发现了在肉瘤中使用细胞表面波形蛋白(CSV)作为生物标记物在诊断间叶肿瘤循环细胞的实用性。在目前的研究中,我们通过使用针对细胞表面波形蛋白(CSV)的单克隆抗体(84-1)去检测上皮以及间叶肿瘤循环细胞以及cellsearch的方法测试了检测循环肿瘤细胞的灵敏度和特异性,血液来源于处在治疗过程中的58名患有浸润乳腺癌的患者,收集的时间点是随机的。另外,我们同时也检测了通过使用其它的参数包括核表面黏连蛋白的定位以及上皮间叶比来提升检测的灵敏度和特异度。

结果:通过细胞表面波形蛋白(CSV)获得的循环肿瘤细胞数在区分对治疗有反应和未有反应的人群中是有显著差异的(P = 0.0053),而通过cell search方法获得的循环肿瘤细胞计数不同组比较的的P值为0.0564. 循环肿瘤细胞的特异性在将两种方法结合起来时发现是最高的,高于没7.5ml血液中检测到8个循环肿瘤细胞的临界值。

结论:将cell search和CSV方法结合起来的循环肿瘤细胞的技术提供了对治疗反应评估的有效方法并且因此为乳腺癌患者提供了个体化治疗的新途径。

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 楼主| 发表于 2015-1-15 10:59:30 | 显示全部楼层
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发表于 2015-4-1 14:25:01 | 显示全部楼层
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